Saturday, March 31

Two satellite meetings will be offered this year. The registration fees for the
satellite meetings are separate from the Annual Meeting registration.  If you have already completed your meeting registration and want to register for either satellite meeting, contact the Registration Manager at leftwich@faseb.org or 301-634-7010 to modify your registration information.

State-of-the-Art Technologies for Producing High-Quality
Recombinant Proteins in Core Facilities

View Flyer (PDF)

8:15 AM - 4:30 PM

Registration Fee (includes lunch):

$295 members

$370 non members

Topics Include:

• Design of protein constructs for optimal expression

• Efficient methods for generating DNA expression vectors

• Optimization and increasing throughput of expression in E. coli

• Overview of expression of membrane proteins

• Cell-free recombinant protein expression

• Use of protein fusion tags for increasing expression and facilitating purification

• Protein refolding strategies and methods to achieve correct disulfide formation

• Characterization of purified recombinant protein reagents to assure high quality

SPEAKERS:

Dr. James Bryson, Bristol-Myers Squibb, Princeton, NJ - Design of Protein Constructs for Optimal Expression and Efficient Methods for Generating DNA Expression Vectors

Dr. Francis Rajamohan, Pfizer Global Research & Development, Groton, CT - Optimization and Increased Throughput of Expression Via Baculovirus and Insect Cells

Dr. Frederick Boyce, Massachusetts General Hospital, Cambridge, MA - Baculovirus Mediated Gene Transfer into Mammalian Cells

Dr. David Wasilko, Pfizer Global Research & Development, Groton, CT - Titer-less Infected Insect Cell Preservation and Scale-Up (TIIPS)

Dr. Yaeta Endo, Ehime University, Matsuyama, Japan - Cell-Free Recombinant Protein Expression

Dr. John Hawes, Miami University, Oxford, OH - Characterization of Recombinant Protein Reagent to Assure High Quality

Dr. Rebecca Page, Brown University, Providence, RI - Strategies to Maximize Heterologous Protein Expression in E. coli with Minimal Cost

Techniques for Quantitative Proteomics

1:00 PM – 5:00 PM

Registration Fee:

$165 members

$240 nonmembers

Topics Include:

• 2-D gel electrophoresis

• Label-free mass spectrometry-based techniques

• Chemical labeling techniques

• Metabolic labeling

• Software for relative quantification

SPEAKERS

Mark W. Duncan
University of Colorado at Denver and Health Sciences Center, Denver, CO  

Michael J. MacCoss
University of Washington, Genome Sciences, Seattle, WA  

Akhilesh Pandey
The Johns Hopkins University School of Medicine, Baltimore, MD  

Michael Washburn
Stowers Institute for Medical Research, Kansas City, MO